White Grubs and Entomopathogenic nematodes
Entomopathogenic nematodes. Entomopathogenic nematodes can be effective parasites of white grubs. Although they are sometimes as effective as chemical insecticides in laboratory trials, field results have been very inconsistent and failures are common. Reasons for poor field results include insufficient water at time of application, improper selection of nematode species, improper storage and handling of the nematodes, and unsuitable environmental conditions.
Among the available commercial isolates of nematodes, Heterorhabditis bacteriophora is the species recommended for the management of white grubs. They should be applied while grubs are still young and most susceptible and before they have caused significant damage. Follow sampling suggestions above, and apply nematodes in areas where high populations have been confirmed. Many types of pesticide sprayers can be used. Use low pressure (< 300 psi), and remove any screens finer than 50 mesh. A hose-end sprayer or watering can is ideal for small-scale applications. Apply a minimum rate of 1 billion nematodes per acre, regardless of the manufacturer’s directions.
Because nematodes are harmed by ultraviolet light, they should be applied at dusk or on a cloudy, rainy day. The nematodes use the thin film of water surrounding soil particles for movement. Irrigation (1/4 inch) after a nematode application is thereby suggested to optimize soil conditions and to help move nematodes through the thatch. A light irrigation before the application will also reduce the chances of nematodes sticking to grass blades on the surface. Users are advised to check viability by examining nematodes for movement with a hand lens before application and again in a sample collected from the sprayer output.
you can see the entire section on white grubs here
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